To successfully carry out the subtraction, we need to calculate the intensity ratio between the contaminated spectrum and the solvent spectrum. Whilst the spectrum below corresponds to the solvent which we need to eliminate from the sample. For example, in this spectrum we have a sample contaminated with a solvent.
It is also possible to subtract spectra, but in this case it is important that the user calculates the intensity ratio of both spectra. 'Load Formulas' On pressing 'OK', the result is a new spectrum which will consist of the sum of spectrum B plus two times spectrum A. 'Save Formulas' will allow the user to save formulas, which can then be loaded by using the In this example, we want to add two times spectrum A to spectrum B, so we shall type the formula 'B+2A' in the corresponding cell. This will display the 'Arithmetic' dialog box on screen.Ĭan’t find the Arithmetics button? Try 'View/Customize User Interface/Customize menu bars' and click on the 'Restore All' button. Just select the desired spectra on the Page Navigator (by holding down 'CTRL key' or 'Cmd key' while clicking on each spectrum) and follow the menu 'Advanced/Arithmetic' or 'NMR/Analysis/More tools/ Arithmetic': This tool is also very useful to remove impurities or solvent signals from a spectrum, to analize DEPT experiments and also to subtract FIDs of Selective Experiments (such as NOE or ROE) How to sum or substract spectra?Īpplication of Linear Combinations (sums, subtraction and multiplication) to spectra in the MestRe Nova interface is extremely simple. Typically, you will use the arithmetic module implemented in MestReNova to sum 'fid''s acquired under the same measurement conditions in order to improve the signal-to-noise ratio. This feature is used to carry out arithmetic operations with 'fid''s or processed spectra.
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